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Image Search Results
Journal: bioRxiv
Article Title: A Linear Mixed Effects Model for Evaluating Synthetic Gene Circuits
doi: 10.1101/2024.12.30.630778
Figure Lengend Snippet: a . Behavior of OR base gate in E. coli DH5α controlling expression of sfgfp and normalized by a constitutive strain lacking all promoter proteins. Data represents n=44. b . Behavior of OR gate weaker RBS (2,091 proportional relative translation rate) regulating LuxR in E. coli DH5α controlling expression of sfgfp and normalized by a constitutive strain lacking all promoter proteins ( e .). Data represents n=44. c . Behavior of OR gate weaker RBS (1,931 proportional relative translation rate) regulating LuxR in E. coli DH5α controlling expression of sfgfp and normalized by a constitutive strain lacking all promoter proteins ( e .). Data represents n=44. d . Behavior of OR gate weaker RBS (104 proportional relative translation rate) regulating LuxR in E. coli DH5α controlling expression of sfgfp and normalized by a constitutive strain lacking all promoter proteins ( e ). Data represents n=44. e . Behavior of OR gate constitutive strain lacking all promoter proteins. Data represents n=44. f . Schematic describing workflow iteration of the TetR RBS design, test, build cycle. g . Correlation between the predicted translation rate of TetR and the ß estimate describing the fixed difference between ON and OFF gate function. Error bars represent the standard error of ß and the linear regression equation is y = 2.5e10 −4 × −0.031.
Article Snippet: Kanamycin sulfate (C18H38N4O15S, Growcells), anhydrotetracycline hydrochloride (aTc, Sigma-Aldrich), 3-oxohexanoyl-homoserine lactone (OC6, Sigma-Aldrich), NEB Gibson Assembly Master Mix (NEB E2611),
Techniques: Expressing